Search results for "Histopathological analysis"

Next-Generation Sequencing Identifies Potential Actionable Targets in Paediatric Sarcomas

2021

Background: Bone and soft-tissue sarcomas represent 13% of all paediatric malignancies. International contributions to introduce next-generation sequencing (NGS) approaches into clinical application are currently developing. We present the results from the Precision Medicine program for children with sarcomas at a reference centre. Results: Samples of 70 paediatric sarcomas were processed for histopathological analysis, reverse transcriptase polymerase chain reaction (RT-PCR) and next-generation sequencing (NGS) with a consensus gene panel. Pathogenic alterations were reported and, if existing, targeted recommendations were translated to the clinic. Seventy paediatric patients with sarcomas…

Clinicopathological and ultrastructural characterization of periapical actinomycosis

2019

Background The aim of the present study was to analyze the clinicopathological and the ultrastructural features of periapical actinomycosis (PA) cases. Material and Methods Data from the files of an oral pathology laboratory were retrieved and the findings of histopathological analysis were evaluated. Hematoxylin–eosin (HE), a modified Brown & Brenn, and Grocott stains as well as ultrastructural analysis using scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) were utilized. Results Six cases were obtained, 4 females and 2 males, with a mean age of 34 year-old. Two cases were symptomatic, lower teeth and the anterior region were more commonly affected, and all…

Tissue Versus Liquid Biopsy: Opposite or Complementary?

2017

The main pillar of cancer diagnosis has been classically represented by the cyto-/histopathological analysis of cells and tissues. The detection of morphological features of cellular atypia (e.g., altered nuclear/cytoplasmic area ratio; nuclear dysmorphism) and disarranged hierarchical architecture of the tissue (i.e., dysplasia) are funding elements in the diagnosis of malignancies, yet the pieces of information conveyed by these features are often insufficient for the precise identification of a specific cancer histotype, and sometimes they prove faulty [1–6].

In vitro histological evaluation of the surgical margins made by different laser wavelengths in tongue tissues

2016

Background: Lasers have become standard tools for the surgical treatment of oral lesions. The purpose of this study is to determine the surgical margins and histologically evaluate the tissue thermal effects induced by different types of surgical instruments. Material and Methods: Cuts were made in pork tonguesâ mucosa with different lasers (Er:YAG at 2W with and without air / water spray and at 4W with and without air / water spray; CO2 at 3.5W and 7W in pulsed mode and at 7W in continuous mode; the diode laser at 3.5W and boost 3.5W in pulsed mode; Nd:YAG at 6W, 40Hz and electroscalpel at 5W and conventional scalpel as control. Macroscopic and microscopic morphological changes were evalua…

Histopathological analysis of human specimens removed from the injection area of expanded adipose-derived stem cells

2010

Can the lesions induced with actinic keratosis become an enigma for dermatologists? Results of an open perspective study

2017

Different face skin diseases (basal cell carcinoma, actinic keratosis, rosacea, solar elastosis, etc.) could clinically manifest itself as erythematic patches, pimples or plagues. It is very hard to make the clinical exclusion in some cases of these diseases since their characters can partially overlap or certain lesion can mimic another one especially in the cases of skin areas affected with sun. Therefore, the histopathological analysis remains the “golden standard” of the dermatological diagnosis at skin diseases. Our study has shown that certified dermatologists detect actinic keratosis (AK) of face/head skin of I/II levels very well. Verdicts of dermatologists and pathomorfologists are…